Aspirin prevents bone loss with little mechanical improvement in high-fat-fed ovariectomized rats

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• 作者：Sien Lin^a ; ^b ; ^c ; ¹ ; Wayne Y.W. Lee^b ; ^c ; ¹ ; Meiling Huang^d ; ¹ ; Ziwei Fu^a ; Yanlong Liang^a ; Haiyou Wu^a ; Liangliang Xu^b ; ^c ; Chun Wai Suen^b ; Jianping Huang^e ; Tie Wu^a ; Liao Cui^a ; ^{cuiliao@163.com" class="auth_mail" title="E-mail the corresponding author} ; Gang Li^b ; ^c ; ^{gangli@cuhk.edu.hk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Aspirin (PubChem CID ; 2244) ; Calcein (PubChem CID ; 65079) ; Cholesterol (PubChem CID ; 5997) ; Sodium cholate (PubChem CID ; 23668194) ; Propylthiouracil (PubChem CID ; 657298) ; Methyl methacrylate (PubChem CID ; 6658)
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：15 November 2016
• 年：2016
• 卷：791
• 期：Complete
• 页码：331-338
• 全文大小：5261 K

文摘

Obesity and osteoporosis are often concurrently happened in the menopausal women. Obesity in menopausal women is not only related to a high risk of cardiovascular disease, but also results in a detrimental effect on bone health. This study aimed to investigate the effects of aspirin, a popular anti-thrombosis drug, on bone quantity and quality in the high-fat-fed animal model. Adult female rats were subjected to either sham operations or ovariectomized operations. The ovariectomized rats were orally administered with deionized water or standardized high fat emulsion with or without aspirin. All rats were injected with calcein before killed for the purpose of double in vivo labeling. Biochemistry, histomorphometry, micro-computed tomography analysis, mechanical test, and component analysis were performed after 12 weeks. In vitro cell culture was also performed to observe the effect of aspirin in osteogenesis. We found that high fat remarkably impaired bone formation and bone biomechanics. Aspirin treatment significantly prevented bone loss by increasing bone formation. In vitro studies also validated the enhancement of osteogenic differentiation. However, aspirin presented no significant improvement in bone mechanical properties. Component analysis shown aspirin could significantly increase the content of mineral, but had limited effect on the content of collagen. In conclusion, aspirin is beneficial for the prevention of bone loss; meanwhile, it may cause an imbalance in the components of bone which may weaken the mechanical properties. The current study provided further evidence that aspirin might not be powerful for the prevention of fracture in osteoporotic patients.