Canine olfactory ensheathing cells from the olfactory mucosa can be engineered to produce active chondroitinase ABC
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- 作者：Darren Carwardinea ; darren.carwardine@bristol.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Liang-Fong Wongb ; l.wong@bristol.ac.uk" class="auth_mail" title="E-mail the corresponding author ; James W. Fawcettc ; jf108@cam.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Elizabeth M. Muird ; emm1@cam.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Nicolas Grangere ; nicolas.granger@bristol.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Chondroitinase ABC ; Chondroitin sulphate proteoglycan ; Olfactory ensheathing cells ; Olfactory mucosa ; Spinal cord injury ; Gene therapy
- 刊名：Journal of the Neurological Sciences
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：367
- 期：Complete
- 页码：311-318
- 全文大小：1117 K
文摘
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Olfactory ensheathing cells from the mucosa can be genetically modified to secrete chondroitinase ABC.
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Lentiviral transduction of olfactory ensheathing cells did not change their morphology or cell marker expression (p75NGF).
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Chondroitinase ABC production was confirmed using the Morgan-Elson assay and Western blot.