Cytotoxic and antibacterial naphthoquinones from an endophytic fungus, Cladosporium sp.
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- 作者：Md. Imdadul Huque Khan<sup>asup> ; Md. Hossain Sohrab<sup>bsup> ; Satyajit Roy Rony<sup>bsup> ; Fakir Shahidullah Tareq<sup>csup> ; Choudhury Mahmood Hasan<sup>asup> ; Md. Abdul Mazid ; Ph.D.<sup>asup><sup> ; sup><sup>ma.mazid@du.ac.bdsup><sup> ; sup><sup>mhsohrab@bcsir.gov.bdsup><sup> ; sup><sup>mazid_ma@hotmail.comsup>
- 关键词：Anhydrofusarubin (PubChem CID ; 157509) ; Methyl ether of fusarubin (PubChem CID ; 14050831) ; Triton X-100 (PubChem CID ; 5590) ; Hydrogen Peroxide (PubChem CID ; 784)
- 刊名：Toxicology Reports
- 出版年：2016
- 出版时间：2016
- 年：2016
- 卷：3
- 期：Complete
- 页码：861-865
- 全文大小：601 K
- 卷排序：3
文摘
Endophytes have the potential to synthesize various bioactive secondary metabolites. The aim of the study was to find new cytotoxic and antibacterial metabolites from endophytic fungus, Cladosporium sp. isolated from the leaves of Rauwolfia serpentina (L.) Benth. ex Kurz. (Fam: Apocyanaceae).Materials and methodsThe endophytic fungus was grown on potato dextrose agar medium and extracted using ethyl acetate. Secondary metabolites were isolated by chromatographic separation and re-crystallization, and structures were confirmed by 1H NMR, 13C NMR and mass spectroscopic data. The cytotoxicity was determined by WST-1 assay and brine shrimp lethality bioassay, while antibacterial activity was assessed by disc diffusion method.ResultsTwo naphthoquinones, namely anhydrofusarubin (1) and methyl ether of fusarubin (2), were isolated from Cladosporium sp. The isolated compounds 1 and 2, by WST-1 assay against human leukemia cells (K-562) showed potential cytotoxicity with IC50 values of 3.97 μg/mL and 3.58 μg/mL, respectively. Initial screening of crude ethyl acetate extract and column fractions F-8 and F-10 exhibited noticeable cytotoxicity to brine shimp nauplii with LC50 values of 42.8, 1.2 and 2.1 μg/mL, respectively. Moreover, the isolated compound 2 (40 μg/disc) showed prominent activities against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus megaterium with an average zone of inhibition of 27 mm, 25 mm, 24 mm and 22 mm, respectively and the activities were compared with kanamycin (30 μg/disc).ConclusionOur findings indicate that anhydrofusarubin (1) and methyl ether of fusarubin (2) might be useful lead compounds to develop potential cytotoxic and antimicrobial drugs.