1,25-Dihydroxyvitamin D3 induces splenocyte apoptosis and enhances BALB/c mice sensitivity to toxoplasmosis
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- 作者：Rohan Rajapakse ; Marc Mousli ; Alexander W. Pfaff ; Bé ; atrice Uring-Lambert ; Luc Marcellin ; Christian Bronner ; Michaë ; l Jeanblanc ; Odile Villard ; Valé ; rie Letscher-Bru ; Jean-Paul Klein and E
- 关键词：Apoptosis ; 1 ; 25-Dihydroxyvitamin D3 ; Toxoplasma gondii ; Th-1 response
- 刊名：The Journal of Steroid Biochemistry and Molecular Biology
- 出版年：2005
- 出版时间：2005
- 年：2005
- 卷：96
- 期：2
- 页码：179-185
- 全文大小：188 K
文摘
The hormonal form of Vitamin D, 1,25-dihydroxyvitamin D3, is well known for its immunosuppressive, anti-proliferative and pro-apoptotic activities. In the present work, we studied the effect of 1,25-dihydroxyvitamin D3 on Toxoplasma gondii-infected mice. We observed that 1,25-dihydroxyvitamin D3 reduces the survival rate of infected mice by up to 37 % at day 10 post-infection compared to untreated infected mice (P < 0.0001). IFN-γ and IL-12p40 levels were significantly reduced by 1,25-dihydroxyvitamin D3 in infected mice sera indicating an inhibition of Th-1-type cytokines. CD4+ T lymphocyte and splenocyte counts were also reduced following 1,25-dihydroxyvitamin D3 treatment and a marked induction of apoptosis, accompanied with down-regulation of the anti-apoptotic proteins Bcl-2 and Bcl-XL, was observed. The above results indicate that 1,25-dihydroxyvitamin D3 induces splenocyte apoptosis and enhances host susceptibility to toxoplasmosis.