Intravenous lipopolysaccharide-induced pulmonary maturation and structural changes in fetal sheep

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• 作者：Boris W. Kramer ; Andreas Ladenburger ; Steffen Kunzmann ; Christian P. Speer ; Jasper V. Been ; J. Freek van Iwaarden ; Luc J.I. Zimmermann ; Markus Gantert ; Yves Garnier
• 关键词：capillary leakage ; chorioamnionitis ; fetal circulation ; surfactant
• 刊名：American Journal of Obstetrics and Gynecology
• 出版年：2009
• 出版时间：February 2009
• 年：2009
• 卷：200
• 期：2
• 页码：195.e1-195.e10
• 全文大小：1304 K

文摘

Background

Antenatal pulmonary inflammation is associated with reduced risk for respiratory distress syndrome but with an increased risk for bronchopulmonary dysplasia (BPD) with impaired alveogenesis.

Objective

We hypothesized that fetal systemic inflammation induced by intravenous (IV) lipopolysaccharide (LPS) would affect lung development in utero.

Study Design

Twenty-one fetal sheep were instrumented (107 days gestational age). Control fetuses received saline (N = 12) and 9 in the study group received 100 ng of LPS IV 3 days after surgery. Animals were assessed for lung maturation and structure after 3 (N = 5) and 7 (N = 4) days.

Results

Interleukin-6 concentration increased in the bronchoalveolar lavage more than 40-fold 3 days after LPS IV. Processing of pro-surfactant protein (SP)-B to mature SP-B and increased SP-B concentrations were shown 7 days after LPS IV. Deposition of elastin fibers at sites of septation was disturbed within 3 days after LPS IV.

Conclusion

Lung maturation and disturbed lung structure occurred after short-term exposure to fetal inflammation and suggests new targeted therapies for BPD.