Evidence of alternative splicing of the chi2 chitinase gene from Metarhizium anisopliae

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• 作者：Juliano Tomazzoni Boldo ; Karina Bohrer do Amaral ; Angela Junges ; Paulo Marcos Pinto ; Charley Christian Staats ; Marilene Henning Vainstein ; Augusto Schrank
• 关键词：Entomopathogenic fungi ; Intron retention ; Endochitinase chi2 ; Transcripts of chi2
• 刊名：Gene
• 出版年：2010
• 出版时间：15 August 2010
• 年：2010
• 卷：462
• 期：1-2
• 页码：1-7
• 全文大小：661 K

文摘

Metarhizium anisopliae is a filamentous fungus used in the biological control of arthropods and produces several chitinases in order to break the host cuticle chitin fibers. Chitinase function during fungal cell development and/or infection processes is also an important aspect when analyzing the life cycle of entomopathogens. The expression profile analysis of the endochitinase chi2 gene acquired by RT-PCR experiments indicated the presence of two different transcripts, suggesting the occurrence of alternative splicing in the chi2 gene. The presence of two transcripts, characterized by the removal or retention of the second 72 bp intron, was further confirmed by DNA sequencing, Northern blot and qRT-PCR. Furthermore, we detected the synthesis of two different proteins from the transcripts by two-dimensional Western blot and mass spectrometry analyses. This is the first reported occurrence of alternative splicing in M. anisopliae.