Leukotriene B₄ inhibits neutrophil apoptosis via NADPH oxidase activity: Redox control of NF-¦ÊB pathway and mitochondrial stability

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• 作者：Pedro Barcellos-de-Souza ; Cl¨¢udio Canetti ; Christina Barja-Fidalgo ; Maria Augusta Arruda
• 关键词：12-HHT ; 12(S)-hydroxyheptadeca-5Z ; 8E ; 10E-trienoic acid ; BLT ; leukotriene B4 receptor ; DPI ; diphenyleneiodonium ; ¦¤¦·m ; mitochondrial transmembrane potential ; ECL ; enhanced chemiluminescence system ; ERK ; extracellular signal-regulated kinase ; FITC ; fluor
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular Cell Research
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：1823
• 期：10
• 页码：1990-1997
• 全文大小：690 K

文摘

Leukotriene B₄, an arachidonic acid-derived lipid mediator, is a known proinflammatory agent that has a direct effect upon neutrophil physiology, inducing reactive oxygen species generation by the NADPH oxidase complex and impairing neutrophil spontaneous apoptosis, which in turn may corroborate to the onset of chronic inflammation. Despite those facts, a direct link between inhibition of neutrophil spontaneous apoptosis and NADPH oxidase activation by leukotriene B₄ has not been addressed so far. In this study, we aim to elucidate the putative role of NADPH oxidase-derived reactive oxygen species in leukotriene B₄-induced anti-apoptotic effect. Our results indicate that NADPH oxidase-derived reactive oxygen species are critical to leukotriene B₄ pro-survival effect on neutrophils. This effect also relies on redox modulation of nuclear factor kappaB signaling pathway. We have also observed that LTB₄-induced Bad degradation and mitochondrial stability require NADPH oxidase activity. All together, our results strongly suggest that LTB₄-induced anti-apoptotic effect in neutrophils occurs in a reactive oxygen species-dependent manner. We do believe that a better knowledge of the molecular mechanisms underlying neutrophil spontaneous apoptosis may contribute to the development of more successful strategies to control chronic inflammatory conditions such as rheumatoid arthritis.