The impacts of handling and air exposure on immune parameters, gene expression, and susceptibility to vibriosis of European abalone Haliotis tuberculata

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• 作者：Marion Cardinaud ; Cl茅ment Offret ; Sylvain Huchette ; Dario Moraga ; Christine Paillard
• 关键词：Abalone ; Stress ; Immunity ; Gene expression ; Vibriosis
• 刊名：Fish & Shellfish Immunology
• 出版年：January, 2014
• 年：2014
• 卷：36
• 期：1
• 页码：1-8
• 全文大小：785 K

文摘

Wild or farmed abalone are regularly exposed to stressors, such as air exposure and handling. Immune and transcriptional responses as well as susceptibility to vibriosis of sexually mature or immature European abalone acclimated at 16 or 19聽掳C were determined following handling or air exposure. Hemocyte density and H₂O₂ production increased while hemocyte viability and phagocytic index decreased following handling. Air exposure induces a decrease of hemocyte density and phagocytic index. Measurement of the expression of genes implicated in general metabolic, immunological and stress responses in gills, foot-muscle and hemocytes by real time q-PCR suggested that both stressors lead to a metabolic rate depression, characterized by a general inhibition of transcription. Finally, following handling a Vibrio harveyi challenge enhances almost 100% mortality of sexually immature animals at 19聽掳C while it has been previously demonstrated that only mature are susceptible to vibriosis.