Soluble squalamine tablets for the rapid disinfection of home nebulizers of cystic fibrosis patients

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• 作者：Lamia Djouhri-Bouktab ; Kamel Alhanout ; Vé ; ronique Andrieu ; Nathalie Stremler ; Jean Christophe Dubus ; Didier Raoult ; Jean Marc Rolain ; ^{jean-marc.rolain@univ-amu.fr} ; Jean Michel Brunel ; ^{bruneljm@yahoo.fr}
• 关键词：Nebulizer ; Cystic fibrosis (CF) ; Aminosterol derivatives ; Disinfection ; Squalamine
• 刊名：Journal of Cystic Fibrosis
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：11
• 期：6
• 页码：555-559
• 全文大小：290 K

文摘

Background

The bacterial contamination of nebulizers represents a major problem for cystic fibrosis (CF) patients that can lead to reduced nebulizer performance and increase the risk of patient reinfection by the contaminating bacteria.

Objective

We investigated the potential use of squalamine, a broad-spectrum antimicrobial compound, as a nebulizer disinfectant.

Methods

Pari LC nebulizers were artificially contaminated with a suspension of bacteria (Staphylococcus aureus and Pseudomonas aeruginosa; 10⁸ CFU/mL) and fungi (Candidida albicans and Aspergilus niger; 10⁷ CFU/mL) and then disinfected by immersion in squalamine solution for 20 min. Glutaraldehyde and Korsolex peracetic acid were used as disinfectant controls.

Result

We found that 0.5 g/L squalamine reduced the levels of viable S. aureus and P. aeruginosa by 5 log₁₀ and the level of viable C. albicans by 4 log₁₀ after 20 min. A concentration of 2 g/L was needed to reduce the level of A. niger cells by 4 log₁₀ in 6 hours. Finally, a formulation of squalamine in the form of a soluble disinfecting tablet containing 2.5 % (w/w) squalamine was developed and successfully applied for nebulizer disinfection.

Conclusion

Our results suggest that aminosterol derivatives may be used by CF patients for rapid and easy home nebulizer disinfection and that soluble tablets may be developed for this purpose.