Synthesis of a 4-nitroimidazole indocyanine dye-conjugate and imaging of tumor hypoxia in BALB/c tumor-bearing female mice

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• 作者：Akram Abuteen^a ; Feifei Zhou^a ; Christopher Dietz^b ; Innus Mohammad^b ; Michael B. Smith^b ; ^{michael.smith@uconn.edu" class="auth_mail" title="E-mail the corresponding author} ; Quing Zhu^a ; ^{zhu@eng.uconn.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Hypoxia ; Cancer ; Synthesis ; Fluorescence ; Dye ; Nitroimidazole
• 刊名：Dyes and Pigments
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：126
• 期：Complete
• 页码：251-260
• 全文大小：1870 K

文摘

Extending our previous work with 2-nitroimidazole-indocyanine dye-conjugate trong class="boldFont">4trong>, we prepared the 4-nitroimidazole-piperazine-indocyanine derivative (trong class="boldFont">6trong>). We also prepared imidazole derivative trong class="boldFont">5trong> as a control. We compared the in vivo hypoxia targeting performance of both trong class="boldFont">5trong> and trong class="boldFont">6trong> with the previously tested trong class="boldFont">4trong>, and trong class="boldFont">6trong> showed a higher fluorescent intensity after 15 min post-injection, about 1.5-fold higher than trong class="boldFont">4trong> and 2.5-fold higher than trong class="boldFont">5trong>. Cells treated with trong class="boldFont">6trong> under hypoxic conditions showed a higher fluorescence yield when compared to the cells kept under normoxic conditions. All findings were supported with fluorescence images of histological sections of tumor samples using a Li-COR scanner and an immunohistochemistry technique for tumor hypoxia.