Investigations of TB vaccine-induced mucosal protection in mice

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• 作者：Azra Blazevic ; Christopher S. Eickhoff ; Jaime Stanley ; Mark R. Buller ; Jill Schriewer ; Eric M. Kettelson ; Daniel F. Hoft
• 关键词：Mycobacterium tuberculosis (Mtb) ; BCG ; Vaccination ; Mucosal specific immunity
• 刊名：Microbes and Infection
• 出版年：January, 2014
• 年：2014
• 卷：16
• 期：1
• 页码：73-79
• 全文大小：766 K

文摘

A better understanding of mucosal immunity is required to develop more protective vaccines against Mycobacterium tuberculosis. We developed a murine aerosol challenge model to investigate responses capable of protecting against mucosal infection. Mice received vaccinations intranasally with CpG-adjuvanted antigen 85B (Ag85B/CpG) and/or Bacillus Calmette-Guerin (BCG). Protection against aerosol challenge with a recombinant GFP-expressing BCG was assessed. Mucosal prime/boost vaccinations with Ag85B/CpG and BCG were protective, but did not prevent lung infection indicating more efficacious mucosal vaccines are needed. Our novel finding that protection correlated with increased airway dendritic cells early post-challenge could help guide the development of enhanced mucosal vaccines.