Preparation of phenylboronic acid-silica hybrid monolithic column with one-pot approach for capillary liquid chromatography of biomolecules

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• 作者：Zian Lin ; ^{zianlin@fzu.edu.cn} ; ^{zalin@nankai.edu.cn} ; Hui Huang ; Shihua Li ; Juan Wang ; Xiaoqing Tan ; Lan Zhang ; Guonan Chen ; ^{gnchen@fzu.edu.cn}
• 关键词：Capillary liquid chromatography ; Organic&ndash ; inorganic hybrid monolith ; 4-Vinylphenylboronic acid ; Tryptic digest ; Proteins
• 刊名：Journal of Chromatography A
• 出版年：2013
• 出版时间：4 January, 2013
• 年：2013
• 卷：1271
• 期：1
• 页码：115-123
• 全文大小：1024 K

文摘

A phenylboronic acid-silica hybrid monolithic column for capillary liquid chromatography (cLC) was prepared through one-pot process by using 4-vinylphenylboronic acid (VPBA) and alkoxysilanes simultaneously. The effects of the molar ratio of tetramethyloxysilane/¦Ã-methacryloxypropyltrimethoxysilane (TMOS/¦Ã-MAPS), amount of VPBA, and the volume of diethylene glycol (DEG) on the morphologies, permeabilities and pore properties of the prepared VPBA-silica hybrid monolithic columns were studied in detail. A relatively uniform monolithic structure with high porosity was obtained with optimized ingredients. A series of cis-diol-containing compounds, alkylbenzenes, amides, and anilines were utilized to evaluate the retention behaviors of the VPBA-silica hybrid monolithic column. The result demonstrated that the prepared VPBA-silica hybrid monolithic column exhibited multiple interactions including hydrophobicity, hydrophilicity, as well as cation exchange apart from the expected affinity interaction. The run-to-run, column-to-column and batch-to-batch reproducibility of the VPBA-silica hybrid monolith were satisfactory with the relative standard deviations (RSDs) less than 1.63 % (n = 5), 2.02 % (n = 3) and 2.90 % (n = 5), respectively, indicating the effectiveness and practicability of the proposed method. In addition, the VPBA-silica hybrid monolithic column was further applied to the separation of proteins and tryptic digest of bovine serum albumin (BSA), respectively. The successful applications suggested the potential of the VPBA-silica hybrid monolith in proteome analysis.