The importance of system band broadening in modern size exclusion chromatography

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• 作者：Alexandre Goyon ; Davy Guillarme ; Szabolcs Fekete ; ^{szabolcs.fekete@unige.ch}
• 关键词：Size exclusion chromatography ; Kinetic efficiency ; Extra-column band broadening ; Aggregates ; Monoclonal antibodies
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：20 February 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：50-60
• 全文大小：1882 K
• 卷排序：135

文摘

150 × 4.6 mm SEC columns cannot be operated on conventional systems. With mAbs, only 15–40% of the column potential is utilized on regular HPLC. HPLC systems also have a huge impact on the apparent elution time. A further decrease of SEC column dimension is not desired.