Identification of a triacylglycerol lipase in the diatom Phaeodactylum tricornutum
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- 作者：Frederik Barkaa ; Max Angstenbergera ; Tilman Ahrendtb ; Wolfram Lorenzenb ; Helge B. Bodeb ; c ; Claudia Bü ; chela ; c.buechel@bio.uni-frankfurt.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：ASP ; artificial seawater provasoli ; ATGL ; adipose triacylglycerol lipase ; BSA ; bovine serum albumin ; DW ; dry weight ; EPA ; eicosapentaenoic acid ; FAME ; fatty acid methyl ester ; IMAC ; immobilized metal affinity chromatography ; IPTG ; isopropyl-β-d-1-thiogalactopyranoside ; PMSF ; phenylmethylsulfonyl fluoride ; PNBP ; para-nitrophenylbutyrate ; qRT-PCR ; quantitative real time polymerase chain reaction ; SDP1 ; Sugar Dependent 1 ; TAG ; triacylglycerol ; TLC ; thin layer chromatography
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：1861
- 期：3
- 页码：239-248
- 全文大小：1221 K
文摘
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Tgl1 is a key enzyme of the lipid catabolism of the diatom alga P. tricornutum.
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The complete coding sequence of Tgl1 is presented.
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Heterologously overexpressed protein shows esterase function.
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Knock-down of this enzyme in P. tricornutum leads to TAG accumulation.
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Overall fatty acid composition is specifically affected by the mutation.