Determination of enantiomeric vigabatrin by derivatization with diacetyl-l-tartaric anhydride followed by ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry
A novel method for enantioselective quantification of vigabatrin was developed. A tartaric acid anhydride was effective for producing vigabatrin diasteromers. Chiral derivatization reaction conditions were optimized by experimental design. Only 20 μL of serum was consumed for sensitivity comparable to literature. First use of UHPLC-Q-TOF MS to quantify vigabatrin in biological samples.