Bioactivity-based UPLC/Q-TOF/MS strategy for screening of anti-inflammatory components from Cimicifugae Rhizoma

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• 作者：Zeng-Yong Wang^a ; Qian Wang^a ; Yan-Qi Han^b ; Min Jiang^a ; Jie Gao^a ; ^{gaojie@nankai.edu.cn} ; Yan Miao^c ; Gang Bai^a
• 关键词：Cimicifugae Rhizoma ; Pseudomonas aeruginosa ; Pneumonia ; NF-κB ; Caffeic acid derivatives
• 刊名：Chinese Chemical Letters
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：28
• 期：2
• 页码：476-481
• 全文大小：744 K
• 卷排序：28

文摘

In this study, the protective effects of Cimicifugae Rhizoma (CR) was demonstrated in Pseudomonas aeruginosa-induced pneumonia mouse model. To identify the anti-inflammatory ingredients, an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) integrated nuclear factor κB (NF-κB) luciferase reporter assay screening system was carried out. As a result, some caffeic acid derivatives, including caffeic acid, ferulic acid/isoferulic acid, fukinolic acid, and cimicifugic acid ingredients were identified as the potential effective compounds from CR. For testing the anti-inflammatory capacity, caffeic acid was demonstrated to inhibit NF-κB and reduce the levels of IL-6 and IL-8 in TNF-α-treated BEAS-2B cells in a dose-dependent manner. Hence, CR preparations and its cinnamic acid derivatives have the possibility to be developed as a complementary therapy in the treatment of respiratory system infection in clinics.