Development and validation of reverse phase high performance liquid chromatography for citral analysis from essential oils

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• 作者：Roopa Gaonkar^a ; ^b ; S. Yallappa^b ; B.L. Dhananjaya^c ; Gurumurthy Hegde^b ; ^{murthyhegde@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：RP-HPLC ; Geranial and neral ; Gas chromatography ; PDA detector ; Mass selective detector
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2016
• 出版时间：15 November 2016
• 年：2016
• 卷：1036-1037
• 期：Complete
• 页码：50-56
• 全文大小：1559 K

文摘

Citral is a widely used monoterpene aldehyde in aromatherapy, food and pesticide industries. A new validated reverse phase high performance liquid chromatography (RP – HPLC) procedure for the detection and quantification of cis-trans isomers of citral was developed. The RP-HPLC analysis was carried out using Enable C – 18G column (250 × 4.6 mm, 5 μ), with acetonitrile and water (70: 30) mobile phase in isocratic mode at 1 mL/min flow. A photodiode array (PDA) detector was set at 233 nm for the detection of citral. The method showed linearity, selectivity and accuracy for citral in the range of 3–100 μg/mL. In order to compare the new RP-HPLC method with the available methods, one of the commercially available essential oil from Cymbopogon flexuosus was analyzed using new RP-HPLC method and the same was analyzed using GC–MS for the comparison of the method for the detection of citral. The GC–MS analysis was done using mass selective detector (MSD) showed citral content to be of 72.76%; wherein the new method showed to contain that same at 74.98%. To prove the application of the new method, essential oils were extracted from lemongrass, lemon leaves and mosambi peels by steam distillation. The citral content present in the essential and also in the condensate was analyzed. The method was found to be suitable for the analysis of citral in essential oils and water based citral formulations with a very good resolution of its components geranial and neral.