Cytotoxic activity and phytochemical standardization of Lunasia amara Blanco wood extract

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• 作者：Muhammad Sulaiman Zubair¹ ; ^{sulaiman_zubair80@yahoo.co.id" class="auth_mail" title="E-mail the corresponding author} ; Syariful Anam¹ ; Subehan Lallo²
• 关键词：Lunasia amara ; Anticancer ; HeLa ; T47D ; Total alkaloid ; Lunacrine
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：6
• 期：11
• 页码：962-966
• 全文大小：646 K

文摘

To evaluate the cytotoxic activity of wood extracts of Lunasia amara Blanco (L. amara) and to perform further phytochemical standardization.

Methods

The wood extracts of L. amara were assessed for cytotoxic activity by in vitro tetrazolium bromide (MTT) method against two human cancer cell lines, cervical cancer cells (HeLa) and breast cancer cells (T47D). Thin layer chromatography, Dragendorf, acetic anhydride-sulfuric acid and ferric chloride were used to detect alkaloids, steroids and polyphenols, respectively. Furthermore, quantitative determination of total alkaloid by ultra fast liquid chromatography-photodiode array detection using lunacrine as a marker compound was performed as well.

Results

The ethyl acetate extract exhibited higher cell-growth inhibition than methanol and n-hexane extracts on HeLa and T47D cancer line cells with the IC₅₀ of 71.15 and 79.04 μg/mL, respectively. Total alkaloid in ethyl acetate extract was counted as (10.46 ± 0.28)% (w/w), while lunacrine determined by ultra fast liquid chromatography-photodiode array detection method was found to be (3.55 ± 0.26)% (w/w).

Conclusions

The high total alkaloid and lunacrine concentration on the extract confirm the potential cytotoxic property of ethyl acetate wood extract of L. amara.