Inversion of type of separation system in planar chromatography of peptides, using C18 silica-based adsorbents
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- 作者：Radosław Ł. Gwardaa ; radoslaw.gwarda@umlub.pl" class="auth_mail" title="E-mail the corresponding author ; Monika Aletańska-Kozakb ; Dariusz Matosiukb ; Tadeusz H. Dzidoa
- 关键词：Thin-layer chromatography ; Peptide separation ; Inversion of separation system type ; Mixed mode chromatography ; Retention mechanism
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：1440
- 期：Complete
- 页码：240-248
- 全文大小：2947 K
文摘
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RP-18 silica-based stationary phases enables to perform RP or NP chromatography of peptides.
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Inversion of separation system type from RP to NP (and vice versa) is explained.
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Scale of the inversion phenomenon is reflected and its application is proposed.
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Variables responsible for the inversions are investigated.
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Change of retention mechanism and selectivity of separation is explained