Preparation of xylooligosaccharides from xylan by controlled acid hydrolysis and fast protein liquid chromatography coupled with refractive index detection
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- 作者：Zhen-Yan Yanga ; 1 ; Ding-Tao Wua ; 1 ; Cun-Wu Chenb ; 1 ; Kit-Leong Cheonga ; Yong Denga ; Ling-Xiao Chena ; Bang-Xing Hanb ; Nai-Fu Chenb ; Jing Zhaoa ; zhaojing.cpu@163.com" class="auth_mail" title="E-mail the corresponding author ; Shao-Ping Lia ; b
- 关键词：Xylooligosaccharides ; Controlled acid hydrolysis ; Fast protein liquid chromatography-refractive index detection ; Anion exchange chromatography ; Size exclusion chromatography
- 刊名：Separation and Purification Technology
- 出版年：2016
- 出版时间：17 October 2016
- 年：2016
- 卷：171
- 期：Complete
- 页码：151-156
- 全文大小：592 K
文摘
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An FPLC-RID coupled with AEC and SEC method was firstly used for preparation and purification of high purity XOSs.
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The XOSs with degree of polymerization 2–6 from xylan were separated and purified.
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The XOSs was rapid analyzed by HPTLC and HPLC-CAD.
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The XOSs were further identified by MALDI-TOF-MS and GC–MS analysis.