Retention behavior of lipids in reversed-phase ultrahigh-performance liquid chromatography-electrospray ionization mass spectrometry
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- 作者：Magdalé ; na Ovčačí ; ková ; Miroslav Lí ; sa ; Eva Cí ; fková ; Michal Holčapek ; Michal.Holcapek@upce.cz" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lipids ; Lipidomics ; Ultrahigh-performance liquid chromatography ; Mass spectrometry ; Human plasma ; Retention behavior
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：1450
- 期：Complete
- 页码：76-85
- 全文大小：2542 K
文摘
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RP-UHPLC/MS two sub–2 μm C18 columns used for study of retention behavior of lipids.
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HR-MS/MS in both polarity modes with high mass accuracy used for identification.
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Method is applied for human plasma, human erythrocytes, human urine and porcine brain.
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Over 400 lipids from 14 classes and 5 lipid categories are unambiguously identified.
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Retention times vs. fatty acyl chain length or DB number fitted by polynomial regressions.