Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics
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- 作者：Grace R. Williamsa ; Jennifer R. Betharda ; Mary N. Berkawa ; Alexis K. Nagela ; b ; Louis M. Luttrellc ; d ; Lauren E. Balla ; b ; ballle@musc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：CID ; collision induced dissociation ; ETD ; electron transfer dissociation ; FTMS ; Fourier transform mass spectrometry ; GO ; Gene Ontology ; GPCR ; G protein-coupled receptor ; GSEA ; geneset enrichment analysis ; HpH RPLC ; high pH reversed phase liquid chromatography ; IPA ; Ingenuity® ; Systems Pathways Analysis ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; MS ; mass spectrometry ; OGE ; off gel electrophoresis ; PTH ; parathyroid hormone ; PTH1R ; type 1 parathyroid hormone receptor
- 刊名：Methods
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：92
- 期：Complete
- 页码：36-50
- 全文大小：3467 K
文摘
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Dynamic phosphorylation events in osteoblasts stimulated with hPTH(1-34) were measured.
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Acute stimulation of the PTH1R engaged canonical GPCR signaling pathways.
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Novel signaling intermediates stimulated by PTH[1-34] were revealed.
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Quantitative LC–MS/MS methods were established to assess PTH1R signaling networks.