Sampling only ten microliters of whole blood for the quantification of poorly soluble drugs: Itraconazole as case study

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• 作者：Justine Thiry^a ; Brigitte Evrard^a ; Gwenaë ; l Nys^b ; Marianne Fillet^b ; Miranda G.M. Kok^b ; ^{miranda.kok@ulg.ac.be}
• 关键词：ACN ; acetonitrile ; BCS ; biopharmaceutics classification system ; DBS ; dried blood spot ; FA ; formic acid ; FDA ; food and drug administration ; HPLC ; high performance liquid chromatography ; IPA ; isopropanol ; ITZ ; itraconazole ; MeOH ; methanol ; MRM ; multiple reaction monitoring ; MS ; mass spectrometry ; OH-ITZ ; hydroxy-itraconazole ; TFA ; trifluoroacetic acid ; UHPLC ; ultra-high performance liquid chromatography ; UV ; ultraviolet ; VAMS ; volumetric absorptive microsampling
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：6 January 2017
• 年：2017
• 卷：1479
• 期：Complete
• 页码：161-168
• 全文大小：950 K
• 卷排序：1479

文摘

Quantification of itraconazole using only 10 μL of whole blood. The method has been validated and the stability of itraconazole has been assessed. The presented method is suited to study the bioavailability of drug formulations. Volumetric absorptive microsampling diminishes the number of required study animals.