High efficient chromogenic catalysis of tetramethylbenzidine with horseradish peroxidase immobilized magnetic nanoparticles
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- 作者：S. Kim1 ; J. Lee1 ; S. Jang ; H. Lee ; D. Sung ; J.H. Chang ; jhchang@kicet.re.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bioimmobilization ; Biocatalysis ; Enzyme activity ; Absorption ; Horseradish peroxidase ; Magnetic nanoparticles
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：105
- 期：part_PB
- 页码：406-411
- 全文大小：1250 K
文摘
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The binding capacity of HRP onto Si-MNPs is 5 ± 1.5 μg per 1 mg of Si-MNPs.
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Chromogenic catalysis of TMB was evaluated by HRP@Si-MNPs and free HRP.
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Apparent kinetic constant (Vmax) of immobilized HRP was 260 μmol/(min mg protein).
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The obtained Vmax was 88-folds higher than the free HRP.