Large tumor suppressors 1 and 2 regulate Aurora-B through phosphorylation of INCENP to ensure completion of cytokinesis

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• 作者：Norikazu Yabuta ; Kaori Yoshida ; Satomi Mukai ; Yorika Kato ; Kosuke Torigata ; Hiroshi Nojima ; ^{snj-0212@biken.osaka-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cell biology
• 刊名：Heliyon
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：2
• 期：7
• 全文大小：6043 K

文摘

The tumor suppressor kinases LATS1 and LATS2 (LATS1/2) regulate not only organ size through the Hippo signaling pathway, but also cell-cycle checkpoints and apoptosis via other signaling cascades. We previously reported that LATS1/2 localize to the mitotic apparatus, where they are involved in the phosphorylation and activation of the mitotic kinase Aurora-B; however, the detailed mechanism of LATS1/2 action remains obscure. The activity of Aurora-B is stringently regulated by formation of the chromosomal passenger complex containing the inner centromere protein (INCENP), which leads to appropriate activation of Aurora-B during mitosis and cytokinesis. In this study, we found that LATS1/2 phosphorylated INCENP at S894 in the Thr-Ser-Ser motif. Moreover, the LATS-mediated phosphorylation of S894 was necessary and sufficient for the activation of Aurora-B, which is required for completion of cytokinesis in cells engaged in multipolar division. We propose a novel mechanism for regulation of Aurora-B via INCENP phosphorylation by LATS1/2 during cytokinesis.