Structural and functional study of ChuY from Escherichia coli strain CFT073

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• 作者：Hun Kim^a ; ² ; Akhilesh Kumar Chaurasia^a ; ² ; Truc Kim^a ; Jongkeun Choi^b ; Sung Chul Ha^c ; Doyoun Kim^a ; ¹ ; Kyeong Kyu Kim^a ; ^{kyeongkyu@skku.edu}
• 关键词：chuY ; Crystal structure ; Flavin reductase ; Heme/iron homeostasis ; Hypothetical protein ; Uropathogenic E. coli CFT073
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：22 January 2017
• 年：2017
• 卷：482
• 期：4
• 页码：1176-1182
• 全文大小：1530 K
• 卷排序：482

文摘

Crystal structure of ChuY of uropathogenic E. coli was determined at 2.4 Å resolution. ChuY has high structural homology with human biliverdin and flavin reductase. ChuY has flavin mononucleotide (FMN) reductase activity using NADPH as a cofactor. A chuY knockout strain showed reduced survival potential in mammalian cells. ChuY plays a role in heme homeostasis and the virulence potential of E. coli CFT073.