Extensive characterization of a lentiviral-derived stable cell line expressing rabbit hemorrhagic disease virus VPg protein

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• 作者：Jie Zhu ; Qiuhong Miao ; Yonggui Tan ; Huimin Guo ; Chuanfeng Li ; Zongyan Chen ; Guangqing Liu ; ^{liugq@shvri.ac.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：VPg ; Rabbit hemorrhagic disease virus ; Cell line
• 刊名：Journal of Virological Methods
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：237
• 期：Complete
• 页码：86-91
• 全文大小：1600 K

文摘

Rabbit hemorrhagic disease virus (RHDV) is an important member of the caliciviridae family. Currently, no suitable tissue culture system is available for proliferating RHDV, which limits the study of its pathogenesis. To bypass this obstacle, we established a cell line, RK13-VPg, stably expressing the VPg gene with a lentivirus packaging system in this study. In addition, the recently constructed RHDV replicon in our laboratory provided an appropriate model for studying the pathogenesis of RHDV without in vitro RHDV propagation and culture. Using this RHDV replicon and RK13-VPg cell line, we further demonstrated that the presence of VPg protein is essential for efficient translation of an RHDV replicon. Therefore, the RK13-VPg cell line is a powerful tool for studying the replication and translation mechanisms of RHDV.