Parylene-A coated microplate for covalent immobilization of proteins and peptides

详细信息    查看全文

• 作者：Byung-Jin Jeon ; Moo-Hwan Kim ; Jae-Chul Pyun
• 关键词：Immunoassay ; Peptide ; Covalent immobilization ; Parylene
• 刊名：Journal of Immunological Methods
• 出版年：2010
• 出版时间：28 February 2010
• 年：2010
• 卷：353
• 期：1-2
• 页码：44-48
• 全文大小：239 K

文摘

Various types of microplates made of polystyrene have been widely used for immunoassays. A new microplate suitable for the covalent immobilization of proteins and peptides was developed by thermal deposition of amino-modified parylene (parylene-A) on the microplate. The primary amine groups of the parylene-A was exploited for the covalent coupling of proteins and peptides. The optical transmittance at the wavelength of 400–500 nm was estimated to be suitable for the application to immunoassays. The immobilization efficiency of the parylene-A coated microplate was demonstrated to be far improved in comparison to the conventional microplate by using horseradish peroxidase (HRP), anti-HRP antibody and a peptide with 9-residues as model biomolecules.