A carbon nanotube metal semiconductor field effect transistor-based biosensor for detection of amyloid-beta in human serum

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• 作者：Jeseung Oh ; Gu Yoo ; Young Wook Chang ; Hyung Joon Kim ; Joachim Jose ; Eosu Kim ; Jae-Chul Pyun ; Kyung-Hwa Yoo
• 关键词：Carbon nanotube ; Metal semiconductor field effect transistor ; Biosensor ; E. coli outer membrane with autodisplayed Z-domain ; Amyloid-¦Â ; Alzheimer's disease ; Human serum
• 刊名：Biosensors and Bioelectronics
• 出版年：2013
• 出版时间：15 December, 2013
• 年：2013
• 卷：50
• 期：Complete
• 页码：345-350
• 全文大小：3373 K

文摘

We have developed a carbon nanotube (CNT) film-based biosensor with a metal semiconductor field effect transistor structure (MESFET). A gold top gate was deposited on the middle of the CNT channel and probe antibodies were immobilized on the gold top gate with an antibody-binding protein, protein G or Escherichia coli outer membrane (OM) with autodisplayed Z-domains of protein A. These CNT-MESFET biosensors exhibited a higher sensitivity than the CNT-FET biosensor with probe antibodies immobilized using a chemical linker, since the orientation of immobilized antibodies was controlled by the antibody-binding proteins. In addition, nonspecific binding was effectively inhibited by E. coli OM. Using the CNT-MESFET biosensors with E. coli OM containing Z domain, we detected amyloid-¦Â (A¦Â) in human serum, one of the biomarkers for early diagnosis of Alzheimer's disease. A¦Â at the level of 1 pg/mL in human serum could be measured in real-time and without labeling, which was lower than a limit of detection for plasma A¦Â using an enzyme-linked immune sorbent assay. These results suggested that our CNT-MESFET biosensors might be applicable for an early diagnosis of Alzheimer's disease.