Mechanism of extraordinary DNA digestion by pepsin
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- 作者：Yanfang Zhang ; Chunchuan Li ; Yu Liu ; Xiaoqian Wang ; Ping Dong ; dongping@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xingguo Liang ; liangxg@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pepsin ; Nucleic acids ; Cleavage characteristics ; Apurinic site ; Mechanism
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：25 March 2016
- 年：2016
- 卷：472
- 期：1
- 页码：101-107
- 全文大小：989 K
文摘
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Cleavage characteristics of pepsin on DNA were investigated.
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Pepsin functioned similar as endonuclease, and presented cleavage preferences for purine-rich sequence (or site) and double-stranded DNA.
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Apurinic site located in the cleavage site could dramatically accelerate DNA digestion.
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A cleavage mechanism was discussed.