AFM membrane roughness as a probe to identify oxidative stress-induced cellular apoptosis
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- 作者：Dau-Chung Wang<sup>asup><sup> ; ; sup><sup>wangdc@yuntech.edu.twsup> ; Ken-Yen Chen<sup>bsup><sup> ; ; sup><sup>g9510807@yuntech.edu.twsup> ; Cheng-Hsien Tsai<sup>csup><sup> ; ; sup><sup>p91421004@ntu.edu.twsup> ; Gen-You Chen<sup>bsup><sup> ; ; sup><sup>g9310801@yuntech.edu.twsup> ; Chuan-Hung Chen<sup>asup><sup> ; ; sup><sup>g9811729@yuntech.edu.twsup>
- 关键词：Atomic force microscope ; Cell apoptosis ; Membrane roughness ; Oxidative stress
- 刊名：Journal of Biomechanics
- 出版年：2011
- 出版时间：10 November 2011
- 年：2011
- 卷：44
- 期：16
- 页码：2790-2794
- 全文大小：1290 K
文摘
The morphological change of cellular apoptosis initiates from the change of membrane roughness. In order to identify cellular apoptosis in its early stage, atomic force microscope was adapted to reveal the change of membrane roughness in unprecedented details, providing an image in nanometer-scaled resolution. The mouse monocyte/macrophage cell line RAW 264.7 was the subject studied and subjected to apoptotic induction by hydrogen peroxide. A finding of the qualitative correlation between cell membrane roughness and oxidative stress level is disclosed stating that roughness is increasing with the increasing level of oxidative stress.