Structural characterization and immunomodulatory effect of a polysaccharide HCP-2 from Houttuynia cordata
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- 作者：Bao-Hui Cheng ; Judy Yuet-Wa Chan ; Ben Chung-Lap Chan ; Huang-Quan Lin ; Xiao-Qiang Han ; Xuelin Zhou ; David Chi-Cheong Wan ; Yi-Fen Wang ; Ping-Chung Leung ; Kwok-Pui Fung ; Clara Bik-San Lau
- 关键词：Houttuynia cordata ; Pectic polysaccharide ; HCP-2 ; Structural characterization ; Immunomodulation
- 刊名：Carbohydrate Polymers
- 出版年：15 March, 2014
- 年：2014
- 卷：103
- 期：Complete
- 页码：244-249
- 全文大小：736 K
文摘
Immunomodulation of natural polysaccharides has been the hot topic of research in recent years. In order to explore the immunomodulatory effect of Houttuynia cordata Thunb., the water extract was studied and a polysaccharide HCP-2 with molecular weight of 60,000 Da was isolated by chromatography using DEAE Sepharose CL-6B and Sephacryl S-400 HR columns. The structure characterization of HCP-2 was performed by Fourier transform infrared spectroscopy (FTIR), acidic hydrolysis, PMP derivation, HPLC analysis and nuclear magnetic resonance spectra (NMR). HCP-2 was elucidated as a pectic polysaccharide with a linear chain of 1,4-linked 伪-d-galacturonic acid residues in which part of the 6-carboxyl groups were methyl esterified and part of 2-hydroxyl groups were acetylated. The bioactivity assays showed that HCP-2 could increase the secretions of interleukin-1尾 (IL-1尾), tumor necrosis factor-伪 (TNF-伪), macrophage inhibitory protein-1伪 (MIP-1伪), macrophage inhibitory protein-1尾 (MIP-1尾), and RANTES (regulated on activation, normal T cell expressed and secreted) in human peripheral blood mononuclear cells (PBMCs), which play critical roles in the innate immune system and shape the adaptive immunity. Our results implied that HCP-2 could be an immune enhancer.