Detection of KRAS mutation by combination of polymerase chain reaction (PCR) and EIS sensor with new amino group functionalization

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• 作者：Yi-Ting Lin ; Ji-Dung Luo ; Chiuan-Chian Chiou ; Chia-Ming Yang ; Chung-Yih Wang ; Chien Chou ; Chao-Sung Lai
• 关键词：Electrolyte&ndash ; insulator&ndash ; semiconductor ; KRAS ; PCR ; NH3 plasma treatment
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：186
• 期：Complete
• 页码：374-379
• 全文大小：1303 K

文摘

A capacitive electrolyte-insulator-semiconductor structure was introduced to quantify and distinguish point mutation from the corresponding PCR product of KRAS gene. Si₃N₄ surface with remote NH₃ plasma is proposed to form amino group for DNA probe immobilization. The sensing response was investigated by capacitance-voltage measurement. A linear relationship was found between the reference voltage and DNA concentration from 10^?6 to 10^?9 M. Non-complementary DNA was used for specificity testing, no DNA hybridized with DNA probes and the specificity of EIS structure with NH₃ treatment was confirmed. Compared with APTES silanization and NH₃ plasma treatment, the sample with NH₃ plasma treatment reveal excellent selectivity and specificity, even in the presence of non-complementary DNA. The NH₃ plasma treatment provided suitable method which compatible CMOS technology and could be improved accuracy of bio-sensing in clinical diagnosis.