Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
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- 作者：Zhou ; Yuanxiang ; Chan ; Jack H. ; Chan ; Annie Y. ; Chak ; Regina K.F. ; Wong ; Elaine Y.L. ; Chye ; Mee-Len ; et. al.
- 关键词：NS1 ; gene encoding the non-structural protein NS1 ; MDCK ; Madin&ndash ; Darby canine kidney ; siRNA ; small-interfering RNA ; RNAi ; RNA interference ; PTGS ; post-transcriptional gene silencing ; EYFP ; gene encoding the enhanced yellow fluorescent protein ; Transge
- 刊名：FEBS Letters
- 出版年：2004
- 出版时间：November 19, 2004
- 年：2004
- 卷：577
- 期：3
- 页码：345-350
- 全文大小：317 K
文摘
As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5′-portion of the NS1 transcripts (5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an Agrobacterium strain harboring a 5mNS1-expressing binary vector caused a reduction in 5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases.