Chiral separation of 2-hydroxyglutaric acid on cinchonan carbamate based weak chiral anion exchangers by high-performance liquid chromatography
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- 作者：Carlos Calderó ; n ; Jeannie Horak ; Michael Lä ; mmerhofer ; michael.laemmerhofer@uni-tuebingen.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Enantioselective chromatography ; 2-Hydroxyglutaric acid (2-hydroxyglutarate) ; Oncometabolite ; 2-Hydroxyglutaric aciduria ; Chiral ion-exchangers ; Cinchona alkaloid
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：7 October 2016
- 年：2016
- 卷：1467
- 期：Complete
- 页码：239-245
- 全文大小：1275 K
文摘
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Enantiomer separation of d- and l-2-hydroxyglutaric acid (2-HG) without derivatization was performed.
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Weak chiral anion-exchanger based on quinidine carbamate (Chiralpak QD-AX) gives baseline resolution.
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Inversion of the elution order was possible on corresponding quinine carbamate stationary phase.
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New generic gradient elution screening procedure is proposed.
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Systematic optimization of MS-compatible conditions was carried out providing insight into separation mechanisms.