In vivo residence duration of human growth hormone loaded in nanogels comprising cinnamoyl alginate, cinnamoyl Pluronic F127 and cinnamoyl poly(ethylene glycol)

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• 作者：Dong Youl Yoon ; Jin-Chul Kim ; ^{jinkim@kangwon.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Human growth hormone ; Nanogel ; Pharmacokinetics ; Pharmacodynamics ; In vivo residence
• 刊名：International Journal of Pharmaceutics
• 出版年：2016
• 出版时间：25 July 2016
• 年：2016
• 卷：509
• 期：1-2
• 页码：229-236
• 全文大小：2139 K

文摘

Cinnamoyl alginate, cinnamoyl Pluronic F127 and cinnamoyl poly(ethylene glycol) were self-assembled into nanogel in aqueous phase. The size of nanogel was 100 nm–200 nm on TEM photos. When 1 ml of human growth hormone (hGH) solution (5 mg/ml, pH3.0) was mixed with 4 ml of the nanogel suspension (37.5 mg/ml, pH3.0), 100% of hGH was loaded in the nanogels (corresponding to specific loading of 3.33% (w hGH/w nanogel)). In the in vivo pharmacokinetics study, the substantial blood level of hGH was maintained during the entire experiment period (two weeks) after one-time injection of hGH-loaded nanogel at 0.5 mg hGH/kg body weight, and the area under curve (AUC) obtained with the one-time injection of hGH-loaded nanogel was higher than AUC obtained with daily injections of free hGH at 0.05 mg hGH/kg for 7 days. In the in vivo pharmacodynamics study, the blood level of insulin-like growth factor (IGF-1) increased in 2 h and it was maintained during the entire experiment period (two weeks) after one-time injection of hGH-loaded nanogel at 0.5 mg hGH/kg body weight. The in vivo fluorescence images of mice injected with rhodamine B isothiocyanate-labelled nanogel suspension showed fluorescence intensity up to 96 h after intravenous injection, indicating that the in vivo residence time of the nanogel was greater than 96 h.