Data on human neutrophil activation induced by pepducins with amino acid sequences derived from β2AR and CXCR4

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• 作者：André ; Holdfeldt ; Malene Winther ; Michael Gabl ; Claes Dahlgren ; Huamei Forsman ; ^{huamei.forsman@rheuma.gu.se" class="auth_mail" title="E-mail the corresponding author}
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：8
• 期：Complete
• 页码：411-414
• 全文大小：415 K

文摘

The data described here is related to the research article titled (Gabl et al., 2016) [1]. Pepducins with peptide sequence derived from one of the intracellular domains of a given G-protein coupled receptor (GPCR) can either activate or inhibit cell functions. Here we include data on human neutrophil function induced by pepducins derived from β2AR (ICL3-8) and CXCR4 (ATI-2341), respectively. ICL3-8 exerts neither direct activating effect on the NADPH-oxidase as measured by superoxide release nor inhibitory effect on FPR signaling. ATI-2341 dose-dependently triggers neutrophil activation and these cells were subsequently desensitized in their response to FPR2 specific agonists F2Pal₁₀ and WKYMVM. Moreover, the ATI-2341 response is inhibited by PBP₁₀ and the peptidomimetic Pam-(Lys-betaNSpe)6-NH2 (both are FPR2 specific inhibitors), but not to the FPR1 specific inhibitor cyclosporine H.