A high-throughput mass spectrometry assay to simultaneously measure intact insulin and C-peptide
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- 作者：Steven W. Taylor ; Nigel J. Clarke ; Nigel.J.Clarke@questdiagnostics.com" class="auth_mail" title="E-mail the corresponding author ; Zhaohui Chen1 ; Michael J. McPhaul
- 关键词：C-peptide ; Diabetes ; Immunocapture ; Insulin ; LC&ndash ; MS/MS
- 刊名：Clinica Chimica Acta
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：455
- 期：Complete
- 页码：202-208
- 全文大小：404 K
文摘
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We have developed a multiplexed method to measure insulin and C-peptide
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The peptides are enriched from sera using 2 different monoclonal antibodies
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Analysis is performed by liquid chromatography-tandem mass spectrometry
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Results are readily converted to SI units
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The assay is amenable to standardization.