Transcriptome analysis of genes related to resistance against powdery mildew in wheat-Thinopyrum alien addition disomic line germplasm SN6306
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- 作者：Quanquan Li1 ; Zubiao Niu1 ; Yinguang Bao1 ; Qiuju Tian ; Honggang Wang ; Lingrang Kong ; Deshun Feng ; dsfeng@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bgt ; Blumeria graminis f. sp. tritici ; GISH ; genomic in situ hybridization ; RT-qPCR ; Reverse Transcription-quantitative Polymerase Chain Reaction ; hai ; hours after inoculation ; RPKM ; Reads Per Kilobases per Million reads ; RNA-seq ; RNA sequencing ; BSMV-VIGS ; Barley stripe mosaic virus-virus induced gene silencing
- 刊名：Gene
- 出版年：2016
- 出版时间：15 September 2016
- 年：2016
- 卷：590
- 期：1
- 页码：5-17
- 全文大小：2303 K
文摘
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SN6306 is a wheat-Thinopyrum intermedium alien addition disomic line germplasm.
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The sequencing generated 104,773 unigenes, among them 9909 showing varied expression levels.
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There were 12 of 39 unigenes that were validated to be involved in Bgt resistance.
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Eight unigenes were cloned and showed that they were similar to those from Th. intermedium.
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The RLKs, ent-CPS and AP may play an important role in Bgt resistance.