An investigation into the role of ATP in the mammalian pre-mRNA 3′ cleavage reaction
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- 作者：Asya Khleborodovaa ; b ; Xiaozhou Pana ; Nagaraja N. Nagrea ; Kevin Ryana ; b ; c ; kr107@sci.ccny.cuny.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pre-mRNA processing ; Cleavage and polyadenylation ; 3&prime ; End formation ; ATP
- 刊名：Biochimie
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：213-222
- 全文大小：1204 K
文摘
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ATP can stimulate or inhibit pre-mRNA 3′ cleavage.
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ATP analog testing is consistent with a cleavage factor binding role, not a kinase substrate role.
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AMP-PCP inhibits 3′ cleavage with an IC50 of 300 μM.
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ATP is not needed for hClp1-Pcf11 complex stability.
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Poly(A) polymerase unable to bind ATP stimulates 3′ cleavage.