Biophysical and functional characterization of the human olfactory receptor OR1A1 expressed in a mammalian inducible cell line
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- 作者：Christine Belloir ; Marie-Louise Miller-Leseigneur ; Fabrice Neiers ; Loï ; c Briand ; Anne-Marie Le Bon ; Anne-Marie.Le-Bon@dijon.inra.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Olfactory receptor ; Recombinant expression ; Purification ; Structure ; Ligand binding
- 刊名：Protein Expression and Purification
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：129
- 期：Complete
- 页码：31-43
- 全文大小：2122 K
文摘
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The human olfactory receptor hOR1A1 was expressed in inducible HEK293S cells.
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Immunoaffinity purification and gel filtration were employed to purify the receptor.
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Purified hOR1A1 was properly folded and presents a monomeric and dimeric structure.
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The purified receptor binds odorant molecules with a high affinity.