We generated a gE/gI/TK-deleted PRV variant-based mutant expressing the CSFV E2 gene.
rPRVTJ-delgE/gI/TK-E2 stably expressing the E2 protein was screened.
rPRVTJ-delgE/gI/TK-E2 was safe and immunogenic for pigs.
rPRVTJ-delgE/gI/TK-E2 provided full protection against lethal CSFV or variant PRV challenge.