Jean Claude Mbanya: fighting diabetes in Africa

详细信息    查看全文

• 作者：Joseph J Schatz
• 关键词：Capsaicin ; DNA microarrays ; GADD153/CHOP ; ER stress ; siRNA ; PC-3 cells ; Prostate cancer
• 刊名：The Lancet
• 出版年：2008
• 出版时间：24 May 2008-30 May 2008
• 年：2008
• 卷：371
• 期：9626
• 页码：1742
• 全文大小：52 K

文摘

The effect of capsaicin, main pungent ingredient of hot chilli peppers, in the gene expression profile of human prostate PC-3 cancer cells has been analyzed using a microarray approach. We identified 10 genes that were down-regulated and five genes that were induced upon capsaicin treatment. The data obtained from microarray analysis were then validated using quantitative real-time PCR assays and Western blot analysis. The most remarkable change was the up-regulation of GADD153/CHOP, an endoplasmic reticulum stress-regulated gene. Activation of GADD153/CHOP protein was corroborated by immunofluorescence and Western blot. We then tested the contribution of GADD153/CHOP to protection against capsaicin-induced cell death using RNA interference. Blockage of GADD153/CHOP expression by small interfering RNA, significantly reduced capsaicin-induced cell death in PC-3 cells. Taken together, these results suggested that capsaicin induces the antiproliferative effect through a mechanism facilitated by ER stress in prostate PC-3 cells.