Six modalities of delivering PN were compared for the in?vitro generation of peroxides and 4-hydroxynonenal: 1) MV-AA?L: light-protected (-L) solution containing multivitamin (MV) mixed with amino acids?+?dextrose (AA); 2) MV-AA+L: MV-AA without photo-protection (+L); 3) MV-LIP+L: MV mixed with lipid emulsion (LIP). LIP was a) Intralipid20 % ? or b) Omegaven?. Hepatic markers of oxidative stress (glutathione, F2¦Á-isoprostanes, GS-HNE) and inflammation (mRNA of TNF-¦Á and IL-1) were measured in newborn guinea pigs infused during 4-days with MV-AA+L compounded with Intralipid20 % ? or Omegaven?.
Hydroperoxides and 4-hydroxynonenal were the lowest in MV-AA?L and the highest in MV-LIP+L. MV-AA+L with Omegaven? was associated with the lowest levels of markers of oxidative stress and inflammation.
Compared to Intralipid20 % ?, Omegaven? reduces oxidative stress associated with PN and prevents liver inflammation. These findings offer an alternative strategy to light protection of PN, which in the clinical setting is a cumbersome modality.