The Mammalian Mismatch Repair Pathway Removes DNA 8-oxodGMP Incorporated from the Oxidized dNTP Pool

详细信息    查看全文

• 作者：Claudia Colussi ; Eleonora Parlanti ; Paolo Degan ; Gabriele Aquilina ; Deborah Barnes ; Peter Macpherson ; Peter Karran ; Marco Crescenzi ; Eugenia Dogliotti ; Margherita Bignami
• 关键词：CELLBIO ; DNA ; RNA
• 刊名：Current Biology
• 出版年：2002
• 出版时间：4 June 2002
• 年：2002
• 卷：12
• 期：11
• 页码：912-918
• 全文大小：315 K

文摘

Mismatch repair (MMR) corrects replication errors. It requires the MSH2, MSH6, MLH1, and PMS2 proteins which comprise the MutSα and MutLα heterodimers [1]. Inactivation of MSH2 or MLH1 in human tumors greatly increases spontaneous mutation rates [2]. Oxidation produces many detrimental DNA alterations against which cells deploy multiple protective strategies [3]. The Ogg-1 DNA glycosylase initiates base excision repair (BER) of 8-oxoguanine (8-oxoG) from 8-oxoG:C pairs [4]. The Myh DNA glycosylase removes mismatched adenines incorporated opposite 8-oxoG during replication [5]. Subsequent BER generates 8-oxoG:C pairs, a substrate for excision by Ogg-1. MTH1—an 8-oxodGTPase which eliminates 8-oxodGTP from the dNTP pool—affords additional protection by minimizing 8-oxodGMP incorporation during replication [6]. Here we show that the dNTP pool is, nevertheless, an important source of DNA 8-oxoG and that MMR provides supplementary protection by excising incorporated 8-oxodGMP. Incorporated 8-oxodGMP contributes significantly to the mutator phenotype of MMR-deficient cells. Thus, although BER of 8-oxoG is independent of Msh2, both steady-state and H₂O₂-induced DNA 8-oxoG levels are higher in Msh2-defective cells than in their repair-proficient counterparts. Increased expression of MTH1 in MMR-defective cells significantly reduces steady-state and H₂O₂-induced DNA 8-oxoG levels. This reduction dramatically diminishes the spontaneous mutation rate of Msh2^−/− MEFs.