Triiodothyronine stimulates cystatin C production in bone cells

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• 作者：Christoph Schmid ; ^{christoph.schmid@usz.ch} ; Claudia Ghirlanda-Keller ; Cornelia Zwimpfer ; Evangelos Zoidis¹
• 关键词：Cystatin C ; Dexamethasone ; Osteoblast ; Triiodothyronine
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：9 March, 2012
• 年：2012
• 卷：419
• 期：2
• 页码：425-430
• 全文大小：508 K

文摘

Thyroid hormones increase cystatin C levels in vivo. To study whether 3,3?5-triiodo-l-thyronine (T₃) stimulates the production of cystatin C in vitro, we used a T₃-responsive osteoblastic cell line (PyMS) which can be kept in serum-free culture. We compared the effects of T₃ on cystatin C mRNA expression (by Northern) and on protein release (by Western and ELISA) with those of dexamethasone (dex). Triiodothyronine increased cystatin C mRNA expression and cystatin C accumulation in culture media in a dose- and time-dependent manner, 1.5-fold at 1 nmol/l after 4 d; dex (100 nmol/l) was more potent and increased cystatin C accumulation 3-fold after 4 d. Triiodothyronine but not dex stimulated glucose uptake. Our in vitro findings explain in vivo observations. Triiodothyronine-induced increase in the production of cystatin C may be related to an increased cell metabolism and proteolysis control demand.