A new lysine derived glyoxal inhibitor of trypsin, its properties and utilization for studying the stabilization of tetrahedral adducts by trypsin
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- 作者：Jennifer A. Cleary ; J. Paul G. Malthouse
- 关键词：Z ; benzyloxycarbonyl ; Z-Lys-H ; Z-NHCH2(CH2)4NH3+ ; Z-Lys-COOH ; Z-NHCH((CH2)4NH3+)-COOH ; Z-Lys-COCHO ; Z-NHCH((CH2)4NH3+)-COCHO
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：5
- 期：Complete
- 页码：272-284
- 全文大小：2665 K
文摘
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- <p id="p0005">Z-Lys-COCHO cyclizes forming a Schiff base.p>
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- <p id="p0010">The Schiff base forms an enol that breaks down to form an alcohol.p>
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- <p id="p0015">Z-Lys-COCHO binding to trypsin inhibits this cyclisation reaction.p>
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- <p id="p0020">Z-Lys-COCHO forms a tetrahedral adduct with trypsin.p>
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- <p id="p0025">The effective molarity of the trypsin active site serine hydroxyl is ~25,000 M.p>