Impact of α-defensins1–3 on the maturation and differentiation of human monocyte-derived DCs. Concentration-dependent opposite dual effects

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• 作者：Marta Rodrí ; guez-Garcí ; a ; Harold Oliva ; Nú ; ria Climent ; Maria M. Escribese ; Felipe Garcí ; a ; Thomas M. Moran ; José ; M. Gatell ; Teresa Gallart
• 关键词：Human neutrophil peptides1– ; 3 ; Antimicrobial peptides ; Innate immunity ; Cytokines ; Dendritic cells
• 刊名：Clinical Immunology
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：131
• 期：3
• 页码：374-384
• 全文大小：1204 K

文摘

α-defensins1–3 are potent antimicrobial molecules that also link innate and adaptive immunity, depending on the concentration range. However, their effects on the biology of human DCs remain largely unknown. We analyzed the impact of different concentrations of α-defensins1–3 on the maturation and differentiation of monocyte-derived DCs (MDDCs). Low doses of α-defensins1–3 up-regulated CD83, CD86 and HLA-DR expression, increased TNF-α, IL-1β, IL-12p40, IL-10 and IL-8 secretion, and slightly augmented allostimulatory capacity. By contrast, high doses down-regulated CD86 and HLA-DR expression, TNF-α, IL-1β, IL-12p40 and IL-10 secretion and allostimulatory capacity, whereas strongly up-regulated IL-8. Furthermore, during the MDDC differentiation process, high doses of α-defensins1–3 affected CD14, CD11c and CD86 expression and strongly up-regulated IL-8. Results suggest that α-defensins1–3 might modulate the maturation and differentiation of MDDCs in vivo and therefore could be of special interest in the field of vaccine development.