Cyclodextrins interact with NBD- and BODIPY-labeled phospholipids.

In aqueous buffer, cyclodextrins bind fluorescent phospholipids disturbing their supramolecular organization.

Cyclodextrins extract fluorescent phospholipids from membranes.

The extent of the interaction depends on the cyclodextrin species, the fluorescence moiety and the phospholipid structure.

The data are important for studies using fluorescent phospholipids in conjunction with CDs.