Cell cloning-on-the-spot by using an attachable silicone cylinder
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- 作者：Hong Bum Parka ; b ; 1 ; garasaki47@nate.com" class="auth_mail" title="E-mail the corresponding author ; Wonseok Sona ; c ; 1 ; sonwonseok@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Dong Han Chaed ; dong8art@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Jisu Leea ; egs0522@naver.com" class="auth_mail" title="E-mail the corresponding author ; Il-Woung Kima ; c ; ilwoung@cnu.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Woomi Yanga ; c ; woomizzang@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Jae Kyu Sunge ; jksung69@cnuh.co.kr" class="auth_mail" title="E-mail the corresponding author ; Kyu Lima ; kyulim@cnu.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Jun Hee Leeb ; meek@kimm.re.kr" class="auth_mail" title="E-mail the corresponding author ; Kyung-Hee Kimf ; phone330@cnu.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Jong-Il Parka ; c ; Jipark@cnu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cell cloning ; Single cell cloning ; Silicone-made cloning cylinder ; MDA-MB-231 breast cancer cell line
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：474
- 期：4
- 页码：768-772
- 全文大小：539 K
文摘
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An easy silicone-made cloning tool was designed for the facile and bona fide cloning of interested cells.
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The silicone cylinder was used to establish MDA-MB-231 subclones showing more authentic properties of the cell line.
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MDA-MB-231 subclones were examined to show that the new cloning tool was advantageous in subcloning of the genuine clonality.